Journal: PeerJ

Article Title: Icaritin protects SH-SY5Y cells transfected with TDP-43 by alleviating mitochondrial damage and oxidative stress

doi: 10.7717/peerj.11978

Figure Lengend Snippet: (A) The MMP of SH-SY5Y cells was monitored with the fluorescent probe JC-1 (magnification: 100×, scale bar 100 µm). (B) Quantitative analysis of the MMP in the different groups. (C) Effects of ICT on ATP content in the model cells. The data are shown as the mean ± SD; n = 3 ( a P < 0.05 vs. the control group). (D) Influence of ICT on CytC expression in the model cells. The data are shown as the mean ± SD; n = 3 ( a P < 0.05 vs. the control group, b P < 0.05 vs. the TDP-43 group).

Article Snippet: The membrane was then incubated for 12 h at 4 °C with a GAPDH antibody (1:1000; Proteintech Group), TDP-43 antibody (1:1000; Proteintech Group), and CytC antibody (1:1000; Proteintech Group).

Techniques: Control, Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Combination of Salidroside and Hedysari Radix Polysaccharide Inhibits Mitochondrial Damage and Apoptosis via the PKC/ERK Pathway

doi: 10.1155/2022/9475703

Figure Lengend Snippet: Effects of CSH on markers of apoptosis in HT22 cells. (a) Relative mRNA levels of PKC β , Bcl-2, and Bax. (b) Representative Western blot results and quantitative analysis of PKC β , Bcl-2, Bax, Cyt-C, cleaved caspase-3, and phospho-ERK1/2 (p-ERK1/2) ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: The membranes were blocked with 5% skim milk for 2 h and incubated with primary antibodies anti-Bcl-2 (lot 11o9905, Affinity Biosciences, Cincinnati, OH, USA), anti-Bax (Affinity, lot 44q6915), anti-PKC β (Affinity, lot 69q9084), anti-Cyt-C (Affinity, lot 73c2522), anti-cleaved caspase-3 (Affinity, lot 15z0096), anti-phospho-Erk1/2 (Cell Signaling Technology, lot 24, Boston, USA), anti-ERK1/2 (Servicebio, lot LS193349, Wuhan, China), and anti- β -actin (Affinity, lot: 7) overnight at 4°C.

Techniques: Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Combination of Salidroside and Hedysari Radix Polysaccharide Inhibits Mitochondrial Damage and Apoptosis via the PKC/ERK Pathway

doi: 10.1155/2022/9475703

Figure Lengend Snippet: A putative schematic model of pharmacological mechanisms of CSH increasing cell viability in A β 25–35 -induced HT22 cells. CSH inhibited apoptosis through regulating the PKC/ERK pathway and markers of apoptosis (cleaved caspase-3, Cyt-C, and Bax).

Article Snippet: The membranes were blocked with 5% skim milk for 2 h and incubated with primary antibodies anti-Bcl-2 (lot 11o9905, Affinity Biosciences, Cincinnati, OH, USA), anti-Bax (Affinity, lot 44q6915), anti-PKC β (Affinity, lot 69q9084), anti-Cyt-C (Affinity, lot 73c2522), anti-cleaved caspase-3 (Affinity, lot 15z0096), anti-phospho-Erk1/2 (Cell Signaling Technology, lot 24, Boston, USA), anti-ERK1/2 (Servicebio, lot LS193349, Wuhan, China), and anti- β -actin (Affinity, lot: 7) overnight at 4°C.

Techniques: